Composite

Part:BBa_I716069:Design

Designed by: Austin Day   Group: iGEM07_Berkeley_UC   (2007-06-27)


2X mutant -pTRC-rbs-beta-rbs-alpha L29F/V96W and Asn108Lys/Lys82Asp


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.


Source

p-TRC (Bgl2/Xho1, 4136/1713, Larger) I716067 -Kan-rbs-beta-rbs-alpha (Bgl2/Xho1, 3081/970, Smaller) Ligated Product is I716069 2X mutant -pTRC-rbs-beta-rbs-alpha (Select the [hopefully] bloodly looking colonies)

References